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2007•Journal of Nanchang UniversityRequires access

Determination of Adenosine in Mycelium of Cultured Cordyceps Sinensis Yiqixue Oral Solution by RP-HPLC

HU Hai-shan

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Abstract

A method was proposed to determine adenosine in mycelium of cultured cordyceps sinensis yiqixue oral solution by reversed-phase high performance liquid chromatography.Adenosine could be seperated on Kromasil C18 column,using methanol-acetonitrile-0.01 mol·L-1KH2PO4(5:5:90) as the mobile phase at flow rate 1.0 mL·min-1,and then detected at 260nm.The concentration of adenosine showed a good linear relationship from 8 to 200 μg·mL-1(r= 0.999 8) and the average recovery was 96.8%.

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What this paper is about

A method was proposed to determine adenosine in mycelium of cultured cordyceps sinensis yiqixue oral solution by reversed-phase high performance liquid chromatography.Adenosine could be seperated on Kromasil C18 column,using methanol-acetonitrile-0.01 mol·L-1KH2PO4(5:5:90) as the mobile phase at flow rate 1.0 mL·min-1,and then detected at 260nm.The concentration of adenosine showed a good linear relationship from 8 to 200 μg·mL-1(r= 0.999 8) and the average recovery was 96.8%.

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Available abstract

A method was proposed to determine adenosine in mycelium of cultured cordyceps sinensis yiqixue oral solution by reversed-phase high performance liquid chromatography.Adenosine could be seperated on Kromasil C18 column,using methanol-acetonitrile-0.01 mol·L-1KH2PO4(5:5:90) as the mobile phase at flow rate 1.0 mL·min-1,and then detected at 260nm.The concentration of adenosine showed a good linear relationship from 8 to 200 μg·mL-1(r= 0.999 8) and the average recovery was 96.8%.

Key concepts: Cordyceps, Mycelium, Adenosine, Chromatography, High-performance liquid chromatography, Chemistry, Methanol, Linear relationship

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