Effects and Mechanisms of Zinc on Viability of HepG2 Cell

Objective To explore the effect of Zinc on the viability of HepG2 cells cultured in virto and its mechanism to provide the experiment ground for treating liver cancer. Method The cell vigor was determined by MTT assay,and the Western blot was performed to evaluate the effects of high concentrations on expression of Bax,Bcl-2.The cell cycle and apoptotic changes were studied by the flow cytometry. Results The results showed that Zinc of high concentration restrained the viability of HepG2 cells.Zinc(250 μmol/L) could inhibit the proliferation of HepG2 cells by causing apoptosis after 24 hours.Zn2+ could increase the level of Bax and decrease the level of Bcl-2 and increase the ratios of Bax/Bcl-2 to induce the HepG2 cells apoptosis. Conclusions Zinc of high concentration could inhibit the restrain of HepG2 cells;the mechanism may be that Zinc of high concentration could increase the level of Bax and decrease the level of Bc1-2 to induce the HepG2 cells apoptosis.