Laboratory evaluation of 18 repellent compounds as oviposition deterrents of Aedes albopictus and as larvicides of Aedes aegypti, Anopheles quadrimaculatus, and Culex quinquefasciatus.

Among 18 experimental skin repellent compounds tested at five concentrations in the laboratory as oviposition deterrents against Aedes albopictus. 12 compounds showed significant activity, with median effective dose values ranging from 0.005 to 0.052%. The test compounds also were evaluated in the laboratory as larvicides against laboratory-reared 4th instars of Aedes aegypti, Anopheles quadrimaculatus, and Culex quinquefasciatus with the same concentrations employed for the oviposition deterrency tests against Ae. albopictus. Larval mortality data at 24 and 48 h after treatment indicated that 12 test repellents caused larval mortalities in the range of 67 to 100% against An. quadrimaculatus, 10 compounds induced 55-100% larval mortalities of Ae. aegypti, whereas larval mortality caused by 9 compounds ranged from 74 to 100% against Cx. quinquefasciatus. These mortality data did not fit the linear model of the statistical analysis. However, multiway analysis of variance of these data showed that the repellent compounds, concentrations used, species of mosquitoes, and exposure times affect the degree of larval mortalities.