Cloning and Expression Analysis of a Gibberellin Receptor BnGID1B Gene from Brassica napus L.

Gibberellins(GAs) make up of an important class of plant hormones and growth regulators,which are involved in a variety of plant growth and development of regulation.As the GA receptor,GID1 is an essential component of GA signaling pathway.In the present study,a GA receptor gene,named BnGID1B(GenBank accession HQ589349) was cloned by the method of homologous cloning from Brassica napus L.The cDNA length sequence of its open reading frame(ORF) was 1 077 bp,encoding a protein of 358 amino acid residues with 40 203.4 Da molecular weight and a theoretical isoelectric point of 6.26.Its genomic DNA sequence contains an intron and two exons.Sequence comparison with Arabidopsis GID1B showed that,BnGID1B gene had 86.3% similarity of nucleotide sequence and 91.64% similarity of the amino acid sequence.SYBR Green I real-time quantitative PCR analysis showed that:BnGID1B gene expression varied in different tissues and was affected by different hormone treatment during seedling stage.It is mainly expressed in roots and the expression level in hypocotyls was lower than that in roots,suggesting that the gene has a certain degree of tissue specificity.