2009•Fudan xuebao. Yixue banRequires access

Effects of RNA interference polo-like kinase-1 gene on telomerase activity of human colon cancer cell

Shu Sen Zheng

Abstract

Objective To investigate the role of polo-like kinase-1(PLK1) on proliferation and telomerase activity of human colon cancer cells. Methods After SW480 colon cancer cell were transfected with small interfering RNA(siRNA)against PLK1,the real time RT-PCR and Western blot assay were used to examine PLK1 gene expression in all cancer cells.The proliferation and telomerase activity of cancer cells were determined by MTT and telomeric repeat amplification protocol enzyme-linked immunoadsordent assay(TRAP-ELISA),respectively. Results Expression of PLK1 of SW480 cancer cell transfected with siRNA down-regulated significantly.Transfection of PLK1 siRNA resulted significantly in inhibition of colon cancer cell in vitro.The results from TRAP showed that cancer cells exhibited marked apoptosis,in time-and dose-dependent pattern. Conclusions RNA interference PLK1 could inhibit proliferation through inducing suppressing telomerase activity of human colon cancer cell.

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Key concepts: Telomerase, Transfection, Cell growth, Molecular biology, RNA interference, Small interfering RNA, Biology, Cancer research