The investigation to the effect of radiosensitive enhancement of nimesulide in lung cancer cell line

Objective:To investigate the enhancement of radiosensition of COX-2 inhibitor Nimesulide on lung cancer cell line (A549) in vitro and try to reaserch the possible radiosensitizing mechanisms. Methods:A549 cell line which is in logarithmic phase was devided into four group:control group,drug group,radiation group and case group. The apoptosis and the expression in protein of Bcl-2 and Bax were measured by flow cytometry(FCM). The enhancement of radiosensitising effect was detect by clone formation experiment. Results:Low dose of Nimesulide combined with X-ray can induce the up-regulation of the protein of Bax and inhibit the expression of the protein of Bcl-2,that increased the apoptosis of tumour cell. The difference is significant. Conclusion:It is suggested that Cox-2 inhibitor-Nimesulide enhance the radiosensitivity to the cell line A549 of adenocarcinoma of lung.