Induction and Culture of Carrot SK4-316 Somatic Embryos

The hypocotyl of carrot SK4-316 aseptic plantlets were used as explants for studying the effects of different culture medium formula and conditions on callus induction,somatic embryos and their synchronization by indirect somatic embryogenesis,and influence of different dedifferentiation time,dedifferentiation medium and the time of explants removed from induction culture systems on direct somatic embryogenesis.The results showed that the medium 1/2MS + 2,4-D2.5 mg/L + 6-BA(or KT) 0.5 mg /L + CH 300 mg/L with 3% sucrose and 0.8% agar was good for callus induction;1/2MS + 2,4-D1.25 mg/L + KT0.25 mg/L + 6-BA0.25 mg/L with 3% sucrose was optimum for somatic embryogenesis from callus,and 0.02% ABA promoted the induction of somatic embryos and 0.06% ABA or 15% PEG promoted the maturation of somatic embryos;it was suit for direct somatic embryogenesis from explants which cultured on dedifferentiation solid medium MS + 2,4-D1.0 mg/L for 48 h,and then on liquid medium MS+CH300g/L.Moreover,somatic embryos mutation rate increased gradually with culture time of explants on induction medium.