Exogenous wild-type p53 gene suppressing the growth of human gallbladder cancer GBC-SD cell line

Objective To observe the growth-suppressive effect of exogenous wild-type p53 (wtp53) gene on human gallbladder cancer GBC-SD cell line. Methods Eukaryotic expressing plasmid pCMV-p53 containing human wtp53 gene was introduced by lipofectamine-mediated gene transfection into human gallbladder cancer GBC-SD cell line. The presence and expression of exogenous p53 gene was confirmed by PCR and RT-PCR, the ability of cell proliferation was assessed using the cell counting and the colony form assay;the cell cycle distribution was detected by flow cytometry. Results The introduction and expression of exogenous p53 gene in transfected cell lines was proved. The cell growth rate and the ability to form colony were remarkably decreased in GBC-SD-wtp53. Cloning efficiency for GBC-SD-wtp53 was 3.2%,which was obviously lower than that for control GBC-SD-mutp53 group (28%) and GBC-SD group (29%) (P0.01).The percent of G0/G1,S and G2/M period was (66.12 ± 4.2) % , (32.87±2.15)%, (1.01± 0.37)% respectively. Compared with GBC-SD-mutp53 cell and GBC-SD cell, the percent of G0/G1 period was significantly increased and the percent of G2/M period was obviously decreased ( P 0.01). Conclusion The expression of exogenous wtp53 gene could effectively inhibit the growth of human gallbladder cancer GBC-SD cell lines in vitro.