Effects of cell apoptosis, MDR1 expression and cell cycle on human bladder cancer cell line treated by arsenic trioxide

OBJECTIVE:To observe the effects of cell apoptosis , MDR1 protein expression and cell cycle on human bladder cancer cell BIU-87 treated by arsenic trioxide (As 2O 3).METHODS: The cell growth inhibitory rates of BIU-87 cell were tested by MTT assay with various concentration As 2O 3 and various time. Fas, bcl-2 , MDR1 protein expression and cell cycle changes were detected by flow cytometry after 72 hours treated with various concentration As 2O 3.RESULTS: As 2O 3 effectively inhibited the growth of BIU-87 cell, which was positive correlation with concentration of As 2O 3 and reaction time,P0.05. Fas expression was positive correlation with the increasing concentration and bcl-2 expression was negative so,P0.05. It was negative correlation with the increasing concentration between Fas and bcl-2 expression,P0.05. MDR1 protein expression treated with 1 μmol/L As 2O 3 was higher than control, but it treated with 2 μmol/L and 5 μmol/L was lower than control,P0.05.Cell cycle was arrested in G 0/G 1 phase with the increasing As 2O 3 concentration.CONCLUSIONS: As 2O 3 could significantly inhibit the growth of bladder cancer cells. Inducing cell apoptosis and arrested cell cycle were probably its important action mechanism.