Impacts of Jiazhongxiao on Apoptosis and Peroxidase Activity of the Thyroid Gland

Objective To research the impacts of jiazhongxiao on thyroid apoptosis and thyroid peroxidase(TPO)activity.Methods The thyroid FRTL cell was used and according to the doses of jiazhongxiao,the control(0 mg·mL-1)group,0.2 mg·mL-1 group,0.4 mg·mL-1 group,0.8 mg·mL-1 group and 1.6 mg·mL-1 group were divided.After culturing thyroid cell for 24 h,Hoechst33258 staining was adopted to observe the morphology of apoptosis.The flow cytometer was used to detect the apoptosis index.The modified guaiacol assay was applied to detect TPO activity.Results After medication for 24 h,under fluorescence microscope,the apoptotic body and nucleus fragments were visible.In 0 mg·mL-1 group,0.2 mg·mL-1 group,0.4 mg·mL-1 group,0.8 mg·mL-1 group and 1.6 mg·mL-1 group,the apoptosis rate(%)was 5.367±0.816,7.517±0.646,14.183±0.752,17.033±1.461 and 23.767±0.734 successively.The dose dependence presented.The difference was significant statistically in comparison among groups(P0.05).TPO activity in each group was 9.395±1.79,7.289±1.205,6.175±1.818,4.347±0.948 and 2.93±1.977 GU·mg-1pr×103 successively.The results were reduced with the increasing of the dose.The difference was significant statistically in comparison among groups(P0.05).Conclusion Jiazhongxiao impacts the thyroid function probably through inducing thyroid apoptosis and inhibiting TPO activity.