The effect of Cyclosporin A on sevoflurane postconditioning in focal cerebral ischemia

Objective:Ischemic cerebral injury is a common complication during perioperative period.We found that ischemic postconditioning and various non-ischemic postconditioning(e.g.inhalation anesthetics) could significantly attenuate ischemic cerebral injury.The present study was to investigate the effect of Cyclosporin A on sevoflurane postconditioning in focal cerebral ischemia.Methods:Fifty male SD rats were randomly assigned to five groups (n=10 each): control group(con),1.0MAC group(sevo),1.0MAC+ Cyclosporin A group(sevo+CsA),1.0MAC+ vehicle group(sevo+vehicle)and Cyclosporin A group(CsA).All animals were subjected to right middle cerebral artery occlusion(MCAO) for 120min followed by reperfusion for 72h.The animals in sevo groups were given 1.0MAC sevoflurane inhalation from 20min before to 10min after reperfusion.The animals in CsA groups were given CsA by ICV injection before sevoflurane postconditioning.The neurological deficit scores(NDS) were recorded at 24h,48h,and 72h after reperfusion.Infarct volume percentage was determined after the last NDS assessment.Results:The infarct volume percentage ratio of Sevo, Sevo+CsA,CsA and Sevo+vehicle groups were 0.31±0.04,0.25±0.04,0.30±0.03 and 0.33±0.05,respectively(P0.05 among the groups),which were significantly smaller than con group(0.55±0.05,P0.05).The infarct volume percentage of Sevo+CsA group was 0.25±0.04,which was significantly different from that of Sevo group and that of CsA group(P0.05).NDS of 24h,48h and 72h after reperfusion in Sevo,Sevo+CsA,CsA and Sevo+vehicle groups were significantly higher than that in con group.Conclusion:Both Cyclosporin A and 1.0MAC sevoflurane postconditioning had significant neuroprotective effects on focal cerebral ischemia/reperfusion injury.The combined neuroprotection was superior to individual alone.