Expression of p38 Mitogen-Activated Protein Kinase and Pulmonary Capillary Barrier Injury in Rats with Severe Acute Pancreatitis

Objective To investgate the expression of p38 mitogen-activated protein kinase(p38MAPK) in lung tissue of rats with severe acute pancreatitis(SAP),and to explore the relationship between p38MAPK and pulmonary capillary barrier injury.Methods Forty male and healthy Sprague-Dawley(SD) rats were randomly(random number method) divided into sham operation(SO) group and SAP group,then rats of SAP group were sub-divided into 3,6,12,and 24 h group,each group enrolled 8 rats,respectively.SAP model rats were established by injecting 5% sodium taurocholate solution retrograde into the biliopancreatic duct.ELISA method was used to test the serum tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β),and pathological changes in lung and pancreas tissues were observed by HE staining.Immunohischemistry method was used to detect phosphorylated p38(p-p38) protein and aquaporin 1(AQP1) protein of lung tissues.The expression level of AQP1 mRNA was measured by quantitative real-time PCR.Results Hyperemia,edema,and inflammatory cell infiltration were observed in lung tissues,abundance of necrosis,part gland structure fuzzy or even disappear were observed in pancreas tissues of all 4 time point groups.Compared with SO group,levels of serum TNF-α and IL-1β were significantly higher in 4 time point groups(P0.05).Lower expression level of p-p38 protein was detected in lung tissues of SO group,while in the early stage of SAP(SAP 3 h group),the expression level of p-p38 protein significantly increased,which peaked in 6 h group and was still higher than SO group in 24 h group(P0.05).Compared with SO group,the expression levels of AQP1 mRNA and protein were significantly lower in all 4 time point groups(P0.05),which had negative correlation with the levels of serum TNF-α, IL-1β,and the expression level of p-p38 protein(r=-0.87,P0.05;r=-0.88,P0.05;r=-0.78,P0.05).Conclusion The decrease of AQP1 protein in lung tissue is one of the vital causes for pulmonary capillary barrier injury in SAP,which probably works by the activation of p38MAPK and the excessive release of inflammatory cytokines.