Preparation of PepsinogenIIMonoclonal Antibody and Development of Sandwich ELISA

The purpose of this study was to prepare pepsinogen Ⅱ monoclonal antibody and establish the sandwich ELISA for serum PGⅡ.Balb / c mice were immunized by using pepsinogenⅡthe immune spleen cells were prepared and fused with SP2 / 0.After screening with HAT cultivation medium,indirect ELISA was used to screen positive clones.Stable hybridoma cells with high titer were obtained after cloning.Ascites were prepared and purified.Pepsinogen Ⅱ double antibody sandwich assay was established after antibody pairing test.Four hybridoma cell lines were obtained : 1D8,1C6,2H11,2E3.1D8,2H11were determined as monoclonal antibody in the sandwich ELISA.The monoclonal antibodies were prepared,and the sandwich ELISA for detecting of PG Ⅱ was developed preliminary.