Effects of retinoic acid in the osteogenic induction to dermal fibroblasts and adipose derived stem cells

Objective To explore the effect of retinoic acid(RA) on ALP activity during the osteogenic differentiation of dermal fibroblasts(DFB) and adipose derived stem cells(ADSCs).Method DFB and ADSCs were isolated from suckling mouse aged 5~7 days and cultured in vitro. The DFB were identified by immunofluorescence of vimentin, and the ADSCs were identified by osteogenic differentiation, adipogenic differentiation and cell surface markers detection. Induced by RA and traditional osteogenic liquid separately for2 weeks, ALP activities were measured by OD value and all specimens were stained by alizarin red. Results All the specimens were stained by alizarin red, except the control groups. Compared with the control groups, all the induced groups displayed a higher activity of ALP. It is worth noting that ALP expression is higher induced by RA than induced by traditional osteogenic liquid. But the opposite results were obtained in the ADSCs groups. Conclusion RA is also responsible for osteogenic differentiation of DFB and ADSCs; what is more,the results may be different due to the varieties of cells.