TOXIC EFFECTS OF ACONITINE ON RAT SERTOLI CELLS

[Objective]To study the toxic effects of aconitine on rat Sertoli cells in vitro.[Methods]Sertoli cells were isolated from testis of 18-20 day-old male Sprague Dawley(SD)rats.To obtain more Sertoli cells,cultures were hypotonically treated with Tris-Hcl,and the Sertoli cells were identified with HE,Wright-Giemsa.Sertoli cells were exposed to one of the following treatments:0?5×101?5×102?5×103?5×104 ng/ml aconitine.Cell viability was determined by the MTT,and concentrations of lactate in media were measured by lactate assay kits.[Results]Compare with control group,aconitine induced increase in Sertoli cell viability at 24 h;The low dose of aconitine(5×101?5×102 ng/ml)induced the increase,while the high dose of aconitine(5×103?5×104 ng/ml)induced the decrease in Sertoli cell viability at 48h.Aconitine stimulated the secretion of lactate from sertoli cells at 24 h.The increase rate of secretion amount of lactate descended when aconitine was at the level of 5×104 ng/ml.[Conclusion]The low dose of aconitine can promote the proliferation of Sertoli cell and the secretion amount of lactate while the high dose can inhibit the proliferation and decrease the stimulation on the secretion of lactate.