2006•Biotechnology(Faisalabad)Requires access
Highly Expression of gfp-lacZ Fusion Gene in Clonging Vector pGEM3Z-f(+)
Yufeng Xia, Qian Zhao, YU Jing-juan
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Abstract
Objective:gfp gene was cloned to pGEM3Z-f(+) vector.Methods:gfp gene was obtained by PCR and inserted into pGEM3Z-f(+) vector.One colony of E.coli DH5α containing.Results: The recombinant plasmid effuses yellow and green fluorescence in sunlight.It also form blue colony in presence of substrate X-gal.on LB solid culture medium.Conclusion:This indicates that a gfp-lacZ fusion gene was obtained.The product of the fusion gene not only has GFP activity,but also keeps β-galcosidase activity.