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Cloning,sequence analysis of the major capsid protein gene of newly isolated iridovirus from Rana tigrina

Miao Su

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Abstract

The complete gene of major capsid protein (MCP) of the recently isolated iridovirus from Rana tigrina was amplificated using the primers corresponding to the two sides of the frog virus 3 (FV3) MCP gene and was cloned into the pGEM T easy vector. The MCP gene of the virus was 1 392 bp in size and had much higher identity to FV3 (98%), the type species of the genus Ranavirus, than to lympocystis disease virus (52%), the type species of the genus Lymphocystivirus. Comparison of the MCP sequence here with this of other vertebrate iridoviruses showed that the iridovirus examined here was a new member of the genus Ranavirus.

About this research paper

What this paper is about

The complete gene of major capsid protein (MCP) of the recently isolated iridovirus from Rana tigrina was amplificated using the primers corresponding to the two sides of the frog virus 3 (FV3) MCP gene and was cloned into the pGEM T easy vector. The MCP gene of the virus was 1 392 bp in size and had much higher identity to FV3 (98%), the type species of the genus Ranavirus, than to lympocystis disease virus (52%), the type species of the genus Lymphocystivirus. Comparison of the MCP sequence here with this of other vertebrate iridoviruses showed that the iridovirus examined here was a new member of the genus Ranavirus.

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Available abstract

The complete gene of major capsid protein (MCP) of the recently isolated iridovirus from Rana tigrina was amplificated using the primers corresponding to the two sides of the frog virus 3 (FV3) MCP gene and was cloned into the pGEM T easy vector. The MCP gene of the virus was 1 392 bp in size and had much higher identity to FV3 (98%), the type species of the genus Ranavirus, than to lympocystis disease virus (52%), the type species of the genus Lymphocystivirus. Comparison of the MCP sequence here with this of other vertebrate iridoviruses showed that the iridovirus examined here was a new member of the genus Ranavirus.

Key concepts: Iridovirus, Biology, Ranavirus, Capsid, Cloning (programming), Gene, Virology, Virus

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Cloning,sequence analysis of the major capsid protein gene of newly isolated iridovirus from Rana tigrina — Research Paper | ScholarLens