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2010•Zhongguo dongwu jianyiRequires access

A method of viable but non-culturable bacteria by fluorescence microscopy

Qian Ai-dong

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Abstract

A detection method of viable but non-culturable bacteria by fluorescence microscopy was established with SYTO9 and PI instead of Acridine Orange and Nalidixic Acid,ink instead of Iraq black,in order to simpli-fy operation process and overcome shortcoming including narrow scope of application,inaccurate observation re-sult,and inconvenient drawing materials.The mothod resolved the technical problems in VBNC and improved the level of operation.

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What this paper is about

A detection method of viable but non-culturable bacteria by fluorescence microscopy was established with SYTO9 and PI instead of Acridine Orange and Nalidixic Acid,ink instead of Iraq black,in order to simpli-fy operation process and overcome shortcoming including narrow scope of application,inaccurate observation re-sult,and inconvenient drawing materials.The mothod resolved the technical problems in VBNC and improved the level of operation.

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Available abstract

A detection method of viable but non-culturable bacteria by fluorescence microscopy was established with SYTO9 and PI instead of Acridine Orange and Nalidixic Acid,ink instead of Iraq black,in order to simpli-fy operation process and overcome shortcoming including narrow scope of application,inaccurate observation re-sult,and inconvenient drawing materials.The mothod resolved the technical problems in VBNC and improved the level of operation.

Key concepts: Acridine orange, Nalidixic acid, Fluorescence microscope, Bacteria, Microscopy, Fluorescence, Microbiology, Orange (colour)

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