Liu Xi-me
Abstract
The electroporation conditions of primary porcine fibroblasts were studied using the EGFP-N1 genes as target gene.The results showed that the transfection efficiency was the best when 4 mg/m L p EGFP-N1 was added and it was pulsed 3 ms under 1 200 V/cm voltage.
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The electroporation conditions of primary porcine fibroblasts were studied using the EGFP-N1 genes as target gene.The results showed that the transfection efficiency was the best when 4 mg/m L p EGFP-N1 was added and it was pulsed 3 ms under 1 200 V/cm voltage.
Key concepts: Electroporation, Transfection, Primary cell, Molecular biology, Cell biology, Green fluorescent protein, Gene, Biology