Comparative Study of Two Different Ways of One-lung Ventilation on the Expression of AQP-5 in Rats

Objective To study the effect of two different ways of one-lung ventilation(OLV)on the expression of lung aquaporin protein-5(AQP-5) in rats.Methods Two models of OLV rats were established,and all the rats were divided into four groups.Group A:performing OLV by clamping.Group B:right bronchial intubation OLV.Group A and Group B was divided into A1,A2,A3,and B1,B2,B3 according to different ventilation time.There are two control groups.Group C:total lung ventilation group,which divided into C1,C2,C3 by the corresponding time with the experimental group.Group D:vacuity contrast group.Take the lung tissue for Western blotting,observed the expression of AQP-5.Results The expression of AQP-5 of group A,group B and group C were less than that of group D(P0.05);Right and left sides of each subgroup was no significant difference(P0.05);Compared within each group,there were significant differences among group A,groupB and group C(P0.05);There was no significantly difference among the group A1,B1 and C1(P0.05);group A2 was less than group B2 and group C2(P0.05),and there was no significantly difference between B2 and C2;Group A3 and group B3 were less than group C3(P0.05),Group A3 was less than group B3(P0.05).Conclusion Mechanical ventilation can reduce the expression of rat AQP-5,and time-dependent.Performing OLV by clamping and right bronchial intubation OLV have higher effect on the expression of rat AQP-5 than total lung ventilation,the former is more obvious.