Effects of CD44 antisense oligo deoxyribonucleotides(ASODN) on the expression of surface antigen of the tumor cells and susceptibility of target cells to CD3AK attack

Objective:To study the role and mechanism of CD44 ASODN on expression of surface molecule of tumor cells and susceptibility of target cells to immune cells attack.Methods:CD44 mRNA and bcl-2 mRNA were examined by RT-PCR.The effect of CD44 ASODN on MGC80-3 cells sensitivity to lysis by CD3AK effector cells was examined by MTT assay.The expression of CD44 and Fas was examined by FACS.Results:Treated with CD44 ASODN(1.6 μmol/L),there was a marked reduction of expression of CD44 protein and mRNA on MGC80-3 cells.Expression ratio of Fas was enhanced from 6.69% to 16.81%(P0.01).Expression of bcl-2 mRNA obviously reduced.After treated with CD44 ASODN,the susceptitility of MGC80-3 cells to lysis by CD3AK was enhanced obviously(P0.01) at the different ratio of effector to target and was time and dose dependent.Conclusion:CD44 ASODN can block and inhibit expression of CD44 mRNA and protein,enhance expression of Fas,reduce expression of bcl-2,which agument sensitivity of MGC80-3 cells to lysis by CD3AK effector cells.