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2003•Bulletin of Chinese CancerRequires access

Apoptosis Induced by Artemisinin in K562 Cells

Song Weihua

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Abstract

To investigate the effect of artemisinin(ART)on the induced apoptosis in K562cells and its mechanism in vitro.The inhibition of ART on K562cells were evaluated by means of MTT assay.The morphological changes of ART on K562cells were observed under transmission electron-microscope.Cell apoptosis rate influenced by ART was assessed by flow cytometer.Mitochondrial trans-membrane potential was assessed by dyeing of the Rhodamine(Rh123).The growth of K562cells was inhibited after being acted by ART for48hours in vitro,the IC 50 of ART was26.53μmol/L.Some typically early and final phase changes of apoptosis were revealod under transmission electromicroscope.Apoptosis rate was associated with ART concentration.Transmembrane potential was significantly de-creased after ATR was administered.[Conclusion]ART can inhibit the proliferation of K562cells and in-duce apoptosis,which closely associated with the decreasing of transmembrane potential.

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What this paper is about

To investigate the effect of artemisinin(ART)on the induced apoptosis in K562cells and its mechanism in vitro.The inhibition of ART on K562cells were evaluated by means of MTT assay.The morphological changes of ART on K562cells were observed under transmission electron-microscope.Cell apoptosis rate influenced by ART was assessed by flow cytometer.Mitochondrial trans-membrane potential was assessed by dyeing of the Rhodamine(Rh123).The growth of K562cells was inhibited after being acted by ART for48hours in vitro,the IC 50 of ART was26.53μmol/L.Some typically early and final phase changes of apoptosis were revealod under transmission electromicroscope.Apoptosis rate was associated with ART concentration.Transmembrane potential was significantly de-creased after ATR was administered.[Conclusion]ART can inhibit the proliferation of K562cells and in-duce apoptosis,which closely associated with the decreasing of transmembrane potential.

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Available abstract

To investigate the effect of artemisinin(ART)on the induced apoptosis in K562cells and its mechanism in vitro.The inhibition of ART on K562cells were evaluated by means of MTT assay.The morphological changes of ART on K562cells were observed under transmission electron-microscope.Cell apoptosis rate influenced by ART was assessed by flow cytometer.Mitochondrial trans-membrane potential was assessed by dyeing of the Rhodamine(Rh123).The growth of K562cells was inhibited after being acted by ART for48hours in vitro,the IC 50 of ART was26.53μmol/L.Some typically early and final phase changes of apoptosis were revealod under transmission electromicroscope.Apoptosis rate was associated with ART concentration.Transmembrane potential was significantly de-creased after ATR was administered.[Conclusion]ART can inhibit the proliferation of K562cells and in-duce apoptosis,which closely associated with the decreasing of transmembrane potential.

Key concepts: Apoptosis, Rhodamine 123, Artemisinin, In vitro, Flow cytometry, Chemistry, MTT assay, Membrane potential

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