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[Study on the relationship between postmortem interval and the change of absorbance in vitreous humor of rabbit after death].

Zhi-qiang Gong, Xiaohong Xu, Y G Sun, D W Wang, Xuan Zhou, Feijia Xu, J L Zhou

Abstract

OBJECTIVE: To seek a exact method of estimating Postmortem interval (PMI). METHODS: This study was preformed to investigate the relationship between postmortem interval and absorbance in vitreous humor of rabbit after death. The absorbance in vitreous humor of 48 rabbits after death were investigated with Model 754 spectrophotometer in apt wavelength (420 nm). RESULTS: There exists positive linear regression association between postmortem interval (Y) and absorbance in vitreous humor (X) (r = 0.98327, P < 0.05), during rabbits after death 0 to 72 hours. The formula of linear regression is Y = 453.30 X + 0.75 (Y = postmortem interval = PMI, X = absorbance in vitreous humor). CONCLUSION: The absorbance in vitreous humor can be as reference indicator to estimate PMI within hour 72.

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Key concepts: Absorbance, Linear relationship, Time of death, Linear regression, Ophthalmology, Chemistry, Chromatography, Medicine