Inhibitory Effects of Knockdown of c-Met by Adenovirus-delivered siRNA on Growth of Hepatocellular Carcinoma Cells

Objective To investigate the effects of knockdown of c-Met by adenovirus-delivered siRNA on the growth of hepatocellular carcinoma cells in vitro.Methods c-Met was knocked down by adenovirus-delivered siRNA. The expression of protein was detected by Western blot. Cell proliferation was determined by MTT assay and Cell count experiments. Cell cycle was analyzed by flow cytometry. Cell colony formation ability was assessed by soft agar colony formation assay.Results The adenovirus-delivered siRNA could decrease the c-Met expression of HCCLM3 cells by over 90%. Knockdown of c-Met could arrest HCCLM3 cells at G1/G0 and inhibit the proliferation of HCCLM3 cells by over 30%(P0.01). Furthermore, Knockdown of c-Met could reduce HCCLM3 cells colony formation by 78% (P0.01).Conclusion Knockdown of c-Met expression by adenovirus-delivered siRNA may be a potential approach for hepatocellular carcinoma gene therapy.