The resistance of Candida albicans to common antifungal drugs and its expression of CDR1 and CDR2 gene

Objective:To investigate the drug resistance in clinical Candida albicans strains and the expression of CDR1and CDR2 gene.Methods:There 176 Candida albicans were isolated and cultivated from various specimens and identify and drug susceptibility test was adopted.The drug resistance genes CDR1 /CDR2 were amplified by RT-PCR.Electrophoresis were used to check all PCR products for analysis of Candida albicans CDR1 and CDR2 genes.Results:From 167 strains of Candida,candida albicans,C.glabrata,C.tropicalis,C.krusei were 79.8 %,9.56 %,8.1 %,2.57 %,respectively.Of 217 Candida albicans strains,the resistant rates against tebinaifen was the highest,94.93%.The resistant rates against itroconazole and 5-flucytosine were 15.21% and 5.99%.The drug resistance genes CDR1 and CDR2 were overexpressed in Candida albicans resistant strains.Conclusion:The most common species in the clinical isolates are still Candida albicans,with non-albicans species appears increasing.The drug resistance of Candida albicans to commonly used antifungals shows a ascending trend.The incidences of CDR1 and CDR2 are overexpressed in Candida albicans resistant strains,which are close correlated with clinical Candida albicans resistance.