Separation of one pectinase strain and optimization for it's production of enzymes

A pectinase strain from the nature would was screened and rescreened on the culture dishes,and cultivated in the shake flask for fermentation.The enzyme activity of pectinase strain was also detected in the cultivation process.The initial pH value of fermentation medium,fermentation temperature,optimum innoculation amount and the fermentation period were studied preliminarily.The results show that the pectinase strain has the best enzyme activity when the initial pH value of fermentation medium is 7.0,the culture temperature is 33 ℃,the innoculation amount is 30% and fermentation period is 56 h.The optimized conditions can greatly improve the enzyme activity.