Effects of resveratrol on cell cycle regulatory processes of human lung adenocarcinoma A549 cells and its mechanism

Objective To investigate the anti-cancer activities of resveratrol on human lung adenocarcinoma A549 cells and its molecular mechanism involved.Methods The effects of resveratrol on the growth of human lung carcinoma A549 cell lines were studied by MTT assay.The effect of resveratrol on the cell cycle phase distribution of A549 cells was analyzed using flow cytometry by a propidium iodide method.The effect of resveratrol on the expression of cyclin D1 and p21cip1 protein was studied by Western blotting analysis.Results MTT assay showed that resveratrol could significantly inhibit the growth of human lung adenocarcinoma A549 cells.It inhibited the proliferation of A549 cells in a time and dose dependent manner,and the highest inhibitory rate was(76.54±1.33)% at the concentration of 100μmol/L when cells were cultured for 48h.Meanwhile,different concentrations of resveratrol treated A549 cells for 24h resulted in an increase of G0/G1 phase cells(P0.01).The expression of cyclin D1 protein was decreased in a time dependent manner in A549 cells treated with resveratrol,while p21cip1 protein was increased.Conclusion Resveratrol could significantly inhibit the growth of A549 cells.Resveratrol is able to inhibit the proliferation of A549 cells by arresting the cell cycle at high concentrations.Regulating the levels of cell cycle regulatory proteins may suggest an involvement in the resveratrol-induced cell cycle arrest.