Detection of virulent Salmonella strains using multiplex PCR

To examine the clinical use of Salmonella identification multiplex PCR and study the epidemiology of Salmonella in Shaanxi Province,19 bacteria isolates were collected from the farms.Biochemical assay and mice challenge model were used to identify of the Salmonellas and their virulence,Multiplex PCR were also used to do this work.The results revealed that multiplex PCR results were consistent with biochemical identification results and mice challenge results.Multiplex PCR technology can rapidly and easily identify of Salmonella and its virulence with high sensitivity and specificity,which may provide widely use in the study of Salmonella epidemiology and detection of Salmonella contamination in animal products.