Identification of genetic relationship of almond by SSR markers

In order to study the genetic relationships of almond germplasm resources,the 27 test materials collected from Gansu that part of superior almond cultivars from abroad,part of domestic breeding improved almond varieties and some related species were identified via SSR molecular marker technique.The results showed that there were 45(77.6%)polymorphic fragments in totally 58 polymerase chain reaction fragments.The genetic similarity varied from 0.498 to 0.956 revealed by NTSYS,with an average of0.757.The clustering results of NTSYS showed that the 27 varieties were clustered into 7groups when the genetic distance was 0.738.Most cultivars from America were clustered with‘Zhipi'and‘Jinbian No.2'.‘Duoguo'was clustered with‘Shantao'.Most cultivars from Xinjiang were clustered into a group.‘Xianfeng'‘Jinbian No.1'‘01-1'and ‘Jiazhou No.1'were clustered into each individual group.It is suggested that the genetic relationship between Xinjiang almond and the American almond is far away.‘Zhipi'‘Jinbian No.2'and most cultivars from America have closer genetic relationship,similar to‘Duoguo'and ‘Shantao'.