The expression of myocardial collagen type Iand type III mRNA in rat with pressure overload

Objective To observe the expression of myocardial collagen type ⅠmRNA and Ⅲ mRNA in rat with pressure overload,study the mechanism of regressing left ventricular hypetrtophy,and study the molecular mechanism of regressing myocardial fibrosis.Methods SD male rats were randomly divided into sham operation group and model group.The aorta was clamped above the suprarenal abdominal aortic by silver chip in model group;the changes of left ventricular myocardium collagen pathological coloring and the left ventricular interstitial cardiac tissue ultra microstructure were observed after 8 weeks,RT-PCR was used to detect the gene expression of type α2(Ⅰ)collagen and type α2(Ⅲ)collagen in myocardial tissue.Results The type α2(Ⅰ)collagen mRNA expression of left ventricular myocardial tissue in model group was higher than that of sham operation group(P0.01).The type α2(Ⅲ)collagen mRNA expression of left ventricular myocardial tissue in model group was higher than that in sham operation group(P0.05).Conclusion The main physiopathologic changes of myocardial fibrosis were formed in the rat with pressure overload by abdominal aortic constriction after 8 weeks,this change of myocardial fibrosis maybe related to the upregulation of the gene expression of type α2(Ⅰ)collagen and type α2(Ⅲ)collagen.