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Utility of multiplex fluorescence in situ hybridization in detection of complex chromosomal aberrations in multiple myeloma

Jinwen Huang

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Abstract

Objective To explore the value of multiplex fluorescence in situ hybridization(M-FISH) in the detection of the complex chromosomal aberrations(CCAs) in multiple myeloma(MM).Methods Two MM patients with CCAs were analyzed by combining the conventional cytogenetics(CC) and M-FISH.Results M-FISH confirmed the aberrations which were previously detected by CC,+5,+7,+9,-13,+15,del(6)(q16q26),add(2)(p25),der(4)t(4;?)(p11;?),add(22)(q11);and also found the specific source of add(2)(p25),der(4)t(4;?)(p11;?),add(22)(q11);detected der(6)t(6;17)(q?;?),der(7)t(1;7)(?;q21)×2,del(9)(q21),der(9)t(1;9)(?;q21),del(16p12),which were undetected or unidentified by CC analysis.Conclusions M-FISH has proved to be useful in characterization of the CCAs in MM,and it is an essential method to refine the karyotype analysis.

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Objective To explore the value of multiplex fluorescence in situ hybridization(M-FISH) in the detection of the complex chromosomal aberrations(CCAs) in multiple myeloma(MM).Methods Two MM patients with CCAs were analyzed by combining the conventional cytogenetics(CC) and M-FISH.Results M-FISH confirmed the aberrations which were previously detected by CC,+5,+7,+9,-13,+15,del(6)(q16q26),add(2)(p25),der(4)t(4;?)(p11;?),add(22)(q11);and also found the specific source of add(2)(p25),der(4)t(4;?)(p11;?),add(22)(q11);detected der(6)t(6;17)(q?;?),der(7)t(1;7)(?;q21)×2,del(9)(q21),der(9)t(1;9)(?;q21),del(16p12),which were undetected or unidentified by CC analysis.Conclusions M-FISH has proved to be useful in characterization of the CCAs in MM,and it is an essential method to refine the karyotype analysis.

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Available abstract

Objective To explore the value of multiplex fluorescence in situ hybridization(M-FISH) in the detection of the complex chromosomal aberrations(CCAs) in multiple myeloma(MM).Methods Two MM patients with CCAs were analyzed by combining the conventional cytogenetics(CC) and M-FISH.Results M-FISH confirmed the aberrations which were previously detected by CC,+5,+7,+9,-13,+15,del(6)(q16q26),add(2)(p25),der(4)t(4;?)(p11;?),add(22)(q11);and also found the specific source of add(2)(p25),der(4)t(4;?)(p11;?),add(22)(q11);detected der(6)t(6;17)(q?;?),der(7)t(1;7)(?;q21)×2,del(9)(q21),der(9)t(1;9)(?;q21),del(16p12),which were undetected or unidentified by CC analysis.Conclusions M-FISH has proved to be useful in characterization of the CCAs in MM,and it is an essential method to refine the karyotype analysis.

Key concepts: Fluorescence in situ hybridization, Multiplex, Karyotype, Molecular biology, Fish <Actinopterygii>, Biology, In situ, Cytogenetics

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Utility of multiplex fluorescence in situ hybridization in detection of complex chromosomal aberrations in multiple myeloma — Research Paper | ScholarLens