Effects of high glucose on proliferation,migration,adhesion and secretion of rat late endothelial progenitor cells

AIM: To investigate the effects of high glucose on the proliferation,adhesion,migration and secretion potentials of late endothelial progenitor cells(EPCs) from bone marrow.METHODS: Mononuclear cells were collected from rat bone marrow by density gradient centrifugation and cultured with M199 medium.The early EPCs were identified by DiI-ac-LDL and FITC-UEA-1 double staining.The late EPCs were identified by RT-PCR to detect the expression of von Willebrand factor(vWF) and VE-cadherin.Moreover,the cells were identified by FACS to detect the expression of CD133 and vascular endothelial growth factor receptor-2(VEGFR-2).The 3rd generation of EPCs was harvested and incubated with glucose in a series of concentrations(5,10,20 or 40 mmol/L).The cell proliferation,adhesion,migration and the secretion of chemokines such as monocyte chemoattractant protein-1(MCP-1) and interleukin-8(IL-8) were assayed with MTT,adhesion test,modified Boyden chamber assay and ELISA,respectively.RESULTS: Compared with normal glucose(5 mmol/L)treatment,high glucose(10,20,40 mmol/L) dose-dependently degraded the proliferation and migration of late EPCs(P0.05 or P0.01).At the same time,treatment with glucose at the concentration of 40 mmol/L decreased the adhesion of EPCs(P0.05) and increased the release of MCP-1 and IL-8 by late EPCs.CONCLUSION: High glucose inhibits proliferation,adhesion and migration of late EPCs,and enhances the secretion of inflammatory factors,indicating that the high glucose correlates with the vascular complications of patients with diabetes.