2013•Zhongguo yufang shouyi xuebaoRequires access
Isolation and identification of Brucella in raw milk.
Xinping Yi, Xiaojing Ma, Yan Jinghua, Gu WenXi, Liya Liu, Ye Feng, Tu Er-hong Nuer, Robert Z. Qi
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Abstract
To investigate the prevalence of Brucella in Xinjiang and improve the isolation method,serum samples collected from 3 dairy farms were tested by serum agglutination test(SAT).The results indicated that 3 dairy farms were Brucellosis-positive between 2010 and 2011.Twenty-five raw milk samples were collected from Brucellosis-positive cows based on SAT test and 9 Brucella isolates were isolated by using a modified Brucella selective supplement in the culture,which were confirmed by VirB8-PCR.In addition to the AMOS-PCR assays,the 9 isolates of Brucella were identified as B.abortus(7 isolates) and B.melitensis(2 isolates),which were further classified into B.abortus biovars 3 and B.melitensis biovars 3 by biochemical test.The results showed that the method of rapid isolation and identification of Brucella was useful for detection of Brucella.