Effects of Fu fang Tang Yang You on the formation of proliferation scars of rabbits

Objective: To investigate the effects of Fu Fang Tang Yang You(复方烫疡油) on the formation of proliferation scars at the ventral side of the ear of rabbits and to explore its possible mechanism.Methods: 144 wounds at the ventral side of the ears of 18 New Zealand white rabbits were created,The area of each wound was 1.2cm×1.0cm.The wounded rabbits were randomly divided into two groups(groups A and B).All the wounds on the right ears of the rabbits in group A were treated with FTY;and the wounds on the left with vegetable oil.All the wounds on the right ears of group B were treated with Sulfadiazine silver cream;and the wounds on the left with normal saline.Tissues from the wounds were examined respectively.30d,60d,90d after injury.Each sample was immunohistochemically stained and examined TGF-β1,type of collagenⅠ?Ⅲ and proliferating cell nuclear antigen(PCNA) monoclonal antibody were examined.The apoptosis index was calculated with TUNEL technique.Results: In FTY group,the time of wound healing was reduced by about 1.5 days;The expression of TGF-β1?PCNA in the tissues of the wounds was lower than that of the control group.Meanwhile,the apoptotic index of fibroblasts was obviously higher than that of the control group.Conclusion: FTY can promote wound healing with less scar formation.