Effect of oxidative damage on expression of pigment epithelium derived factors in cultured human retinal pigment epithelial cells

Objective To investigate the effect of oxidative damage on the expression of pigment epithelium derived factor(PEDF)in human retinal pigment epithelial(RPE)cells.Methods Cultured human RPE cells were treated with 600 μmol·L-1 H2O2 for different time periods,2 hours,8 hours and 24 hours.The expression of PEDF protein were studied by immunocytochemistry technique.The PEDF mRNA was studied by reverse transcriptase PCR.Results Strong positive expression of PEDF protein was detected in control group,and PEDF expression gradually decreased at 2 hours,8 hours and 24 hours in H2O2 injury group,presenting a significant difference between H2O2 injury group and control group(P0.05).The changes in PEDF mRNA paralleled to the changes in its protein.Conclusion Oxidative damage could down-regulate the expression of PEDF in human RPE cells,which also related with the action time at a certain extent.