Effect of extracorporeal circulation with autologous lung as oxygenator on CPB-relative inflammatory response

Objective To determine the protective effect of cardiopulmonary bypass(CPB) with autologous lung as oxygenator on CPB-relative inflammatory response.Methods Twelve adult mongrel dogs were randomly divided into control group and experimental group.CPB using a membrane oxygenator (control group) or using the autologous lung (experimental group) for gas exchange was performed for 120 min in an alternating series of 12 mongrel dogs with 90 min heart arrest by crystalloid cardioplegia and 30 min reperfusion.The blood samples were collected at the same time point of pre-operation(T1),60 min of CPB(T2),and 1 h (T3),2 h (T4) after CPB.The plasma levels of IL-6,IL-10,TNF-α were detected with ELISA.Results The plasma levels of IL-6,IL-10 and TNF-α of dogs in each group were significantly increased at T2,T3 and T4(P0.01) compared with before CPB.The plasma levels of IL-6 and TNF-α in experimental group were significantly lower than those in control group at T2,T3 and T4(P0.01).The plasma levels of IL-10 in experimental group were significantly higher than those in control group at T2,T3 and T4(P0.05).Conclusion Extracorporeal circulation with autologous lung as oxygenator could reduce the increased amplitude of the plasma levels of TNF-α and IL-6,whereas enhance the increased amplitude of the plasma IL-10 level.The results indicate that extracorporeal circulation with autologous lung as oxygenator can reduce the CPB-relative inflammatory respones.