The effects of advanced glycosylation end products on the secretion of interleukin-8 in cultured human mesangial cells

Objective To study the effect of advanced glycosylation end products(AGEs)on the expression of interleukin-8(IL-8)in cultured human renal mesangial cells(HRMC).Methods The AGEs-BSA was prepared by incubating bovine serum albumin(BSA)with glucose.The cultured HRMC(10 6)were intervened with the same concentration of BSA and AGEs-BSA(160,320 and 640μg/ml)for 48h,or 640μg/ml for 1,4,8,24 and 48h.The IL-8 level in supernatant was measured by ELISA.Results The IL-8 concentration began to elevate(71.9±9.2pg/ml,P0.05)after 4h intervention with AGEs-BSA,and further increased(546.6±49.7,652.1±42.8 and 2 485.2±99.9pg/ml,P0.01)when HRMC incubated with AGEs-BSA for prolonged time(8?24 and 48h).The level for IL-8 also increased (178.1±15.6,475.4±18.9 and 2 485.2±99.9pg/ml,P0.01)with the increase of AGEs-BSA concentration(160,320 and 640μg/ml).Conclusion The AGEs-BSA can stimulate the secretion of IL-8 in cultured HRMC in concentration and time dependently.