2001•Unpublished venueRequires access

Cloning and expressing of HIV-1gp41N and C pep-tide gene

Qian Zhao

Abstract

Aim To express N and C peptide genes of HIV-1gp41in E.Coli JM109.Methods N and C peptide genes of HIV -1gp41were amplified from plasmid containing c DNA of HIV -1gp160gene by PCR and cloned into prokaryotic expression vector pGEx-4T -1.The cloned genes were subcloned into pGEM7zf(+)and sequenced.Expression of the cloned genes were induced in E.coli JM109and analysed on SDS -PAGE.Results The N and C peptide genes were amplified and cloned successfully,and which was confirm ed by restriction endonuclease and sequence analysis.The fused GST -N a nd GST -C were expressed successfully identified by SDS -PAGE.Conclusion The expression of N and C peptide genes of HIV -1gp41have laid the foundation for further investigation.

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Key concepts: Cloning (programming), Restriction enzyme, Gene, Molecular biology, Biology, Plasmid, Multiple cloning site, Molecular cloning