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Content Determination of Geraniol in Cymbopogon citratus by GC

Xiao Zhi-xing, Feng Jing-yi

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Abstract

OBJECTIVE:To establish the method for the content determination of geraniol in Cymbopogon citratus.METHODS:Geraniol was extracted from C.citratus by ultrasonic extraction.The content of geraniol was determined by GC.Chromatographic column was Agilent Technologies DB-WAX(30 m×0.25 mm×0.25 μm) with PEG-20M as solid phase.The column temperature was 130 ℃,injector temperature was 230 ℃ and temperature of detector was 250 ℃.The flow rated of N2(carrier gas) was 1.0 mL·min-1 kPa.The flow rated of H2(fuel gas) was 40 mL·min-1 and that of air(assistant fuel gas) was 400 mL·min-1.RESULTS:The linear range of geraniol was 0.015 3～0.107 1 μg(r=0.997 3) with an average recovery of 97.8%(RSD=1.68%,n=6).CONCLUSION:This method is simple,rapid and accurate,with good reproducibility.It can be used for quality control of C.citratus.

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OBJECTIVE:To establish the method for the content determination of geraniol in Cymbopogon citratus.METHODS:Geraniol was extracted from C.citratus by ultrasonic extraction.The content of geraniol was determined by GC.Chromatographic column was Agilent Technologies DB-WAX(30 m×0.25 mm×0.25 μm) with PEG-20M as solid phase.The column temperature was 130 ℃,injector temperature was 230 ℃ and temperature of detector was 250 ℃.The flow rated of N2(carrier gas) was 1.0 mL·min-1 kPa.The flow rated of H2(fuel gas) was 40 mL·min-1 and that of air(assistant fuel gas) was 400 mL·min-1.RESULTS:The linear range of geraniol was 0.015 3～0.107 1 μg(r=0.997 3) with an average recovery of 97.8%(RSD=1.68%,n=6).CONCLUSION:This method is simple,rapid and accurate,with good reproducibility.It can be used for quality control of C.citratus.

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Available abstract

OBJECTIVE:To establish the method for the content determination of geraniol in Cymbopogon citratus.METHODS:Geraniol was extracted from C.citratus by ultrasonic extraction.The content of geraniol was determined by GC.Chromatographic column was Agilent Technologies DB-WAX(30 m×0.25 mm×0.25 μm) with PEG-20M as solid phase.The column temperature was 130 ℃,injector temperature was 230 ℃ and temperature of detector was 250 ℃.The flow rated of N2(carrier gas) was 1.0 mL·min-1 kPa.The flow rated of H2(fuel gas) was 40 mL·min-1 and that of air(assistant fuel gas) was 400 mL·min-1.RESULTS:The linear range of geraniol was 0.015 3～0.107 1 μg(r=0.997 3) with an average recovery of 97.8%(RSD=1.68%,n=6).CONCLUSION:This method is simple,rapid and accurate,with good reproducibility.It can be used for quality control of C.citratus.

Key concepts: Cymbopogon citratus, Geraniol, Chromatography, Chemistry, Citral, Reproducibility, Content determination, Essential oil

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