Optimization of Purification Technology for Tea Polyphenol from Tea Stalk by Macroporous Resin

Objective:To optimize purification technology of tea-polyphenol from tea stalk by macroporous resin,and provide a reference for secondary development of tea stalk.Method:Static absorption-desorption and dynamic adsorption-desorption was used to select the best one from 9 different type macroporous resin;With the content of tea-polyphenol as index,purification technology parameters of tea polyphenol were optimized by single factor test.Result:LX-18 type resin showed the best purifying profile,its optimum technology conditions were as follows:crude drugs-dry resin weight 1∶ 1,adsorption rate of 2 mL·min-1,eluted by 10 BV distilled water and 10 BV 50% ethanol;Purity of tea polyphenol was 53.79%.Conclusion:This optimized technology was simple and feasible with good purification effect,so it could provide a reference for development of related products of tea polyphenol.