Determination of AST activity in seminal plasma and methodology evaluation

Objective To establish the method of seminal plasma aspartate aminotransferase(AST)activity determination and the methodology evaluation.Methods The method of seminal plasma AST activity determination was established by the method of serum AST activity detection.According to National Committee for Clinical Laboratory Standards(NCCLS)document EP5-A and EP6-A,its precision and linearity and the difference of the results between different technicians were also observed to evaluate the acceptability of the method.Results The intraassay coefficient of variation(CV)was 1.93%,inter-assay CV was 2.77%,between-day CV was 3.45%,total CV was 4.84% for low level sample.The intraassay CV was 0.48%,inter-assay CV was 0.53%,between-day CV was 0.53%,total CV was 0.89% for high level sample.The linearity was 1652 U/L.There was no significant difference in the AST activity detected by 2 technicians(P=0.423).Conclusion The acceptable method of seminal plasma AST activity determination could be established by the method of serum AST activity detection.