Establishment of Calli-mediated Regeneration System in Curcuma longa

A protocol was investigated for callus induction using bud base from tissue-cultured Curcuma longa explants.The results showed that MS media supplemented with 2,4-D or with 2,4-D+6-BA could successfully induce calli formation at cut positions of explants.However,the calli grew slowly.The optimal calli induction medium was MS+0.5 mg/L TDZ+0.5 mg/L BA+0.3 mg/L 2,4-D,which showed the highest induction rate and calli growing mass,with an inducing rate of 80% and growing mass of 148.6 mg per month.The optimal plantlet regeneration medium was MS+0.5 mg/L TDZ+2.0 mg/L BA+0.2 mg/L NAA,with a regeneration rate of 90% and the average shoot number of 5.1 per explant.Regenerated plantlets(5 cm at length) were transferred to soil in greenhouse.More than 90% of plantlets could survive.