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Detection of the genes encoding oprD2 and metallo-β-lactamases in imipenem-resistant Pseudomonas aeruginosa

WU Da-we

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Abstract

ObjectiveTo evaluate the association between presence of oprD2 and metallo-β-lactamases (MBLs) genes and imipenem resistance.MethodsThe 2-mercaptoethanol (2-MPA) was used as enzyme inhibitor. The synergistic antimicrobial susceptibility testing was done by K-B method with MH agar. The metallo-beta-lactamase genes and OprD2 gene were detected by PCR.ResultsOf the 60 clinical isolates of imipenem-resistant P. aeruginosa (IRPA),15 (25%) were MBL-positive using phenotypic methods,22 (36.36%) positive for oprD2 gene,19 (31.66%) positive for IMP-1 type MBL by PCR. Neither VIM-2 nor IMP-2 type MBL gene was identified among these strains.ConclusionsIMP-1 is the most prevalent MBL type in Tianjin. The IMP-1-producing isolates are an important contributor to imipenem resistance.

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What this paper is about

ObjectiveTo evaluate the association between presence of oprD2 and metallo-β-lactamases (MBLs) genes and imipenem resistance.MethodsThe 2-mercaptoethanol (2-MPA) was used as enzyme inhibitor. The synergistic antimicrobial susceptibility testing was done by K-B method with MH agar. The metallo-beta-lactamase genes and OprD2 gene were detected by PCR.ResultsOf the 60 clinical isolates of imipenem-resistant P. aeruginosa (IRPA),15 (25%) were MBL-positive using phenotypic methods,22 (36.36%) positive for oprD2 gene,19 (31.66%) positive for IMP-1 type MBL by PCR. Neither VIM-2 nor IMP-2 type MBL gene was identified among these strains.ConclusionsIMP-1 is the most prevalent MBL type in Tianjin. The IMP-1-producing isolates are an important contributor to imipenem resistance.

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Available abstract

ObjectiveTo evaluate the association between presence of oprD2 and metallo-β-lactamases (MBLs) genes and imipenem resistance.MethodsThe 2-mercaptoethanol (2-MPA) was used as enzyme inhibitor. The synergistic antimicrobial susceptibility testing was done by K-B method with MH agar. The metallo-beta-lactamase genes and OprD2 gene were detected by PCR.ResultsOf the 60 clinical isolates of imipenem-resistant P. aeruginosa (IRPA),15 (25%) were MBL-positive using phenotypic methods,22 (36.36%) positive for oprD2 gene,19 (31.66%) positive for IMP-1 type MBL by PCR. Neither VIM-2 nor IMP-2 type MBL gene was identified among these strains.ConclusionsIMP-1 is the most prevalent MBL type in Tianjin. The IMP-1-producing isolates are an important contributor to imipenem resistance.

Key concepts: Imipenem, Pseudomonas aeruginosa, Microbiology, Biology, Gene, Antibiotics, Antibiotic resistance, Bacteria

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Detection of the genes encoding oprD2 and metallo-β-lactamases in imipenem-resistant Pseudomonas aeruginosa — Research Paper | ScholarLens