Optimization of the Extraction Process of Flavonoids from Lotus Seed Shell by Using Response Surface Methodology

The process of enzyme-assisted extraction of total flavonoids from the lotus seed shell was optimized.Based on the single-factor tests,the effects of enzyme amount time of enzymatic hydrolysis,p H value and temperature of enzymatic hydrolysis on the extraction yields of total flavonoids from lotus seed shell were studied by response surface methodology(RSM).The results indicated that cellulase showed better ability of enzymatic hydrolysis.The optimum enzyme-assisted extraction process was enzyme amount 6.5 mg / g,time 1.5 h,p H value 4.7 and temperature 46 ℃.The highest yield of total flavonoids was 7.1%.