Bcl-XL siRNA in inhibition of human colon cancer cells growth

Objective: To study the inhibitory effect of a double-strand small interfering RNA targeting Bcl-XL (Bcl-XL siRNA) in human colon cancer cells. Methods: Human colon cancer cell line DLD1 was treated with Bcl-XL siRNA, The protein level of Bcl-XL in the cells was determined by Western blot; the apoptotic ratio and survival condition were detected by flow cytometry assay and trypan blue-stained cell counting with hemocytometer, respectively. Results: The level of Bcl-XL protein in DLD1 cells was obviously down-regulated by Bcl-XL siRNA. The apoptotic ratio was significantly higher in Bcl-XL siRNA treated group than those in control (P0.05) , whereas the survival rate of cells was significantly decreased in Bcl-XL siRNA group(P 0. 05). Conclusion: Bcl-XL siRNA can effectively knock down the Bcl-XL protein and inhibit the growth of human colon cancer cells, which might be a new way for colon cancer treatment.