Studies on Induce Condition,Purification and Partial Character of Extracellular Laccase from Coriolus Versicolor CV-8

The laccase of Coriolus versicolor CV-8 was purified by salting out from ammonium sulfate,dialyzed by semipermeable membrane,DEAE-Cellulose ion exchange and Sephadex G100 column chromatography in order to explore its productive situation and characteristics.It was purified 36.1 times;and the specific activity and recovery of purification laccase were 10180 u/mg and 46.6% respectively.The curve of laccase production and optimal condation of laccase reaction were also studied.The results showed that Coriolus versicolor came the laccase production peak which was 398u/ml at the 11 day in this culture condition.The optimal temperature and optimal pH of laccase reaction were at 25℃ and pH4.6.Mg~(2+),Mn~(2+) and Cu~(2+) increased laccase activity whereas Ag~+,Fe~(3+) and Cl~-obviously inhibited it.The form view Km of laccase was 892×10~(-3)mol/L with o-Tolidine as substrate.This fungus can be the productive strain of the Lignin Degradation and the laccase in envirmental protection industry.