Changes of apoptosis rate and expression of apoptosis -related gene of PPL after burn and delayed resuscitation

Objectives: To study the effects of burn and delayed resuscitation on the apoptosis percentage (ap%) of intestinal Peyer's patch lymphocyte (PPL) and the expression of apoptosis-related genes in rats. Methods:Thirty Wistar rats were divided into early resuscitation(ER) and delayed resuscitation(DR) groups,24 rats were subjected to 30% TBSA III degree scald burn,6 sham rats were served as control. DNA fragmentation, DNA agarose gel electrophoresis were used to observe the changes of ap% ; RT-PCR was applied to exam the mRNA expression of interleukin-1β-converting enzyme (ICE), B-cell lymphoma/leukemia-2 (bcl-2), TNFα and IL-2 postburn. Results: The ap% of PPL increased significantly at 6 h postburn (PB), distinct DNA ladders were discerned in agarose gel electrophoresis. The expression of ICE increased dramatically postburn, and it expressed higher in DR group than in ER group . The expression of bcl-2 was strong in PPL before burn and became weak and weak postburn. The TNFα gene showed a stronger expression and was enhanced by delayed resuscitation. The expression of IL-2 showed a trend of descending; and there was a significant difference at 12 h postburn between the two groups. Conclusion: The imbalance expression of apoptosis-enhanced gene and apoptosis-inhibited gene after burn and delayed resuscitation might be responsible for the dramatically increased apoptosis in PPL.