Cyclic Heptadepsipeptide Inhibited Retinal Neovascularization and Nonperfusion in Oxygen Induced Ischemic Retinopathy Mice

To investigate the effects of two novel fungus derived cyclic heptadepsipeptide (Hep A) and (Hep B) on oxygen induced ischemic retinopathy in mice.The C57BL/6 mice were placed in 75%oxygen from postnatal day (P) 5 to P12. At P12, the mice were put back in room air and treated with subcutaneous injection of 10 mg/kg Hep A, 10 mg/kg Hep B, or vehicle twice a day. At P17, mice were either sacrificed and eye frozen sections were stained with griffonia simplicifolia lectin B4 (GSA) which selectively stained vascular cells and counterstained with eosin; or perfused with fluorescein dextran and then made retinal flat mount. The areas of the retinal neovascularization or retinal nonperfusion were tested under microscopy and recorded by a vadio camera and frame grabber, and then quantitated by Image Pro Plus sofeware. The data were analyzed by ANOVA software.Compared to vehicle treated mice, the area of retinal nonperfusion and neovascularization in oxygen induced ischemic retinopathy mice treated with Hep A or Hep B was significantly reduced.[Conclusion]The analogs of cyclic heptadepsipeptide potently inhibit retinal nonperfusion and neovascularization in oxygen induced ischemic retinopathy mice.