Cryoprotective effect of trehalose in keeping aortic valve homograft

Objective To search for the application of trehalose as cryoprotectant for keeping aortic valve homograft in liquid nitrogen.Methods The aortic valve homografts of Wistar rat were kept in 10%DMSO and divided into 2 groups(control and test group).The test group was added a supplement of trehalose 0.1mol·L~(-1) and all the hemografts were gradually hypothermied and then cryopreserved in-196℃ liquid nitrogen.After 6 months all the cryopreserved valves were thawed.The aortic valve homografts were observed by light and electron microscope.At the same time,the viability of endotheliocyte was measured.Results The viability of endotheliocyte in test group was higher than that in the control group and the structural damage was also much lighter.Conclusion The cryoprotective effect of combined use of 10%DMSO+0.1mol·L~(-1) trehalose was much better than that of 10%DMSO alone.