Changes of the Percentages of γδT Cells and CD_4~+CD_(25)~+Foxp3~+Treg Cells in Peripheral Blood of Multiple Sclerosis Patients

Objective To investigate the changes of the percentages of γδT cells and CD4 +,CD25 +,Foxp3+,Treg cells in the peripheral blood of patients with multiple sclerosis(MS).Methods Peripheral blood mononuclear lymphocytes of 37 cases MS patients(MS group) and 25 cases healthy controls(NC group) were separated with Ficoll Hypaque density gradient centrifugation.The proportion of γδT cells and CD4 +,CD25 +,Foxp3+,Treg cells were detected with flow cytometry.Results The percentage of γδT cells in the peripheral blood of MS patients was significantly elevated compared with the healthy controls,and that of active patients were higher than inactive patients.The percentage of CD4 +,CD25 +,Foxp3+,Treg cells decreased in the peripheral blood of MS patients compared with healthy controls,with it being lower in active patients than inactive patients.There was significant negative correlation between the percent of γδT cells and the percent of CD4 +,CD25 +,Foxp3+,Treg cells in MS patients(P0.05).Conclusion The up-regulation of peripheral blood γδT cells in MS patients might cause the down-regulation of peripheral CD4 +,CD25 +,Foxp3+,Treg cells,thus contributies to the occurrence of the disease.